Stained chromosome suspensions were analyzed on a flow cytometer Mo-Flo, Beckman Coulter as described previously Ng and Carter In Mongolian gerbils, SYCP3 paints the Y chromosome and distal tip of the X, forming a protein bridge between the two sex chromosomes de la Fuente et al. Although some Y-linked recessive disorders exist, typically they are associated with infertility in males and are, therefore, not transmitted to subsequent generations.
Use of a limited non homologous sex chromosomes in North Carolina of starting material can result in strong amplification bias Chen et al. Paint X 3 paints the entire chromosome X 3 as well as the long arm of Y 2 Figure 4fso is the fifth chromosome in the chain. These early mapping results should help in the identification of the primary sex-determining locus, in the investigation of mechanisms of dosage compensation and in understanding the evolution of vertebrate sex chromosomes.
X-linked : Associated with the X chromosome.
For example, positive or negative directional selection can reduce diversity at linked sites, with a greater effect in regions of low recombination, known as hitchhiking and background selection Hill and Robertson, ; Hudson and Kaplan, ; Nordborg et al.
See Figure 6—source data 1, 2. We are currently unable to define where in the pathway this effect is occurring, though our mutant analysis indicates differences in the sensitivity of interfering and non-interfering repair to sequence polymorphism.
Fertilization of these gametes produces individuals with either too many or not enough chromosomes. Genet; Simon et al. Table 8.
The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. As discussed above we did not detect significant trans effects via QTL analysis in these populations and our use of independently derived recombinant lines in later experiments will mitigate against any undetected weak effects from other chromosomes.
We have added additional I3bc experiments Figure 5which address these issues directly and demonstrate that juxtaposition of heterozygous and homozygous regions causes reciprocal increases in recombination in the heterozygous region and reduces them in the homozygous region Figure 5.
Chromosome-wide control of meiotic crossing over in C. We appreciate the need to more fully discuss literature relevant to the relationship between recombination and sequence diversity.
We developed a k-mer based computational strategy to define the set of sequences present in each X and Y flow-sorted pool see Materials and Methods. B Biol. Chromosomes 1, 6, and 27 each are polymorphic and are represented by two peaks.